gst smad3 mh2 (Addgene inc)
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gst smad3 mh2
Gst Smad3 Mh2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+smad3+mh2/pcDNA3-HA-CUL1+delta+C22+(Plasmid+%2319942)/pm22782721-79-0-10
Average 85 stars, based on 1 article reviews
Gst Smad3 Mh2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+smad3+mh2/pcDNA3-HA-CUL1+delta+C22+(Plasmid+%2319942)/pm22782721-79-0-10
Average 85 stars, based on 1 article reviews
gst smad3 mh2 - by Bioz Stars,
2026-09
85/100 stars
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1) Product Images from "Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program."
Article Title: Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program.
Journal: Development (Cambridge, England)
doi: 10.1242/dev.078345
Figure Legend Snippet: Fig. 1. Endogenous Smad3 and JMJD3 interact in NSCs. (A)Size-exclusion chromatography of NSC lysate showing co-elution of Smad3P and JMJD3, and the presence of Smad3 in the lower weight fractions. (B)Co-IP of mouse NSCs lysate using anti-Smad3P antibody or unrelated IgGs in the presence or absence of TGFfor 30 minutes. (C)Upper panel shows schematic representation of GST-Smad3 fragments: full length (FL), MH1 (1-155 aa), MH2 (199-425 aa) and linker domains that also contains MH2 (146-425 aa). Pull-down assay using GST-Smad3 fusion proteins and 293t cell extracts overexpressing Myc-JMJD3. Ponceau staining of GST-Smad3 proteins (lower panel). (D)Immunoblot from control knockdown (C KD) and JMJD3 knockdown (JMJD3 KD) cell extracts using the indicated antibodies. (E)Immunoblot showing Nestin expression prior to and after TGFtreatment for the indicated times in C KD and JMJD3 KD cells. Nestin levels (relative to Actin) were quantified by using the Image J software (graph on the right). Input (In) corresponds to 1% of the protein present in the whole-cell extract.
Techniques Used: Size-exclusion Chromatography, Co-Elution Assay, Co-Immunoprecipitation Assay, Pull Down Assay, Staining, Western Blot, Control, Knockdown, Expressing, Software
Related Articles
Size-exclusion Chromatography:Article Title: Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program. Article Snippet: GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).. Co-Elution Assay:Article Title: Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program. Article Snippet: GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).. Co-Immunoprecipitation Assay:Article Title: Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program. Article Snippet: GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).. Pull Down Assay:Article Title: Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program. Article Snippet: GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).. Staining:Article Title: Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program. Article Snippet: GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).. Western Blot:Article Title: Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program. Article Snippet: GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).. Control:Article Title: Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program. Article Snippet: GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).. Knockdown:Article Title: Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program. Article Snippet: GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).. Expressing:Article Title: Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program. Article Snippet: GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).. Software:Article Title: Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program. Article Snippet: GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).GST-Smad3 full-length and GSTSmad3 MH1 domain (1-155) were kindly provided by Dr J. Massagué (Xu et al., 2003).. |